Coding

Part:BBa_K1331003:Design

Designed by: Jiayi Dong   Group: iGEM14_Nankai   (2014-09-26)


RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221


Design Notes

It includes both the complete coding sequence of RhlI and part of noncoding sequence between RhlR and RhlI gene.

The EcoRI(at 250) and PstI(692) restriction sites have been mutated to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Source

It comes from the genomic sequence of P.aeruginosa SQ6.

References